Other

Part:BBa_K4674012:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)


The exprssing cassette for tetrahedral ssDNA with cis-auto splicing motif


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 212
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 212
    Illegal NheI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 212
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 212
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 271
    Illegal NgoMIV site found at 479
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nope


Source

This cassette comprises RCORI-105, tetraheral ssDNA, cis-autosplicing motif, and RCORI-65.

References